TIMELAPSE VISION INC.

Bacterial phagocytosis by macrophage

The discovery begins.

“To see things beyond ordinary vision”

this is what motivated us to capture the phenomena of life through a microscope.

Based on these microscopic images, we create original video content.

TLV Lab

Images of Experiments

TLV has its own in-house experimental facilities and devices, so we can plan and implement any customized experiment or reproduce microscopic images of any specific phenomenon.

We handle microorganisms, such as cells, bacteria, viruses, or molds, according to Biosafety Level 2 (BSL2, P2).

We also provide supportive experiments, analysis, and photography for conference presentations and thesis submission.

Equipment

Clean bench, safety cabinet, CO2 incubator, constant temperature incubator, autoclave, dry heat sterilizer, gas replacement glove box, ultrapure water system, high-speed refrigerated centrifuge, clean isolator, breeding facility for experimental animals, deep freezer, liquid nitrogen, medical freezer/refrigerator, inverted microscope, and stereomicroscope.

Imaging Microscope

Inverted confocal laser scanning microscope, inverted phase contrast microscope, inverted differential interference contrast microscope, upright biological microscope, upright fluorescence microscope, research fluorescence stereomicroscope, and culture system for microscopy

TLV Conference Presentation / Thesis List

International conference

2018　10th ISCTICO 2018 in Kyoto　　

　　　 Zinc deficiency increases the extracellular ATP release of keratinocytes and exacerbates dermatitis.

　　　- study by imaging -

2016　9th ISCTICO 2016 in Krakow, Poland. A novel in vivo live imaging assay for endothelial nitric oxide

　　　 production revealed improving effects of "Kangen-karyu" on blood flow.

2014　Society For Redox Biology and Medicine (SFRBM) 2014 annual meating in Seattle, USA Aspirin and /or

　　　 NSAIDs-induced intestinal mucosal injury are required primary mucosal dysfunction caused by cellular

　　　 reactive oxygen species production.

2014　8th International Symposium on Cell/Organoprotection 2014 in Budapest, Hungary

　　　 NSAIDS- INDUCED ULCER FORMATIONS OF INTESTINAL MUCOSAE ARE NECESSARY

　　　 BOTH PRIMARY SYSTEMIC DISFUNCTION OF MUCOSAL DEFENSE SYSTEM AND

　　　 SECONDARY LOCAL ATTACK FACTORS

2013　Society for Free Radical Reserch Japan International Free Radicel Winter School in Muikamachi

　　　 Reactive Oxigen make Cancer Dance

2012　American Society for Cell Biorogy (ASCB) 2012 annual meeting in San Fransisco, USA

　　　「Cancer Dance movement」 CellDance contest 3rd place

2012　7th International Symposium on Cell/Tissue Injury and Cytoprotection/Organoprotection in Hawaii,

　　　 USA

　　　 NSAIDs treatments involved ROS generation to induce epithelial cellular injuries in the mice

　　　 intestine before lesions formation

2011　American Society for Cell Biorogy (ASCB) 2011 annual meeting in Denver, USA

　　　「Cancer Dance」 CellDance contest 1st place

2010　17th International The Society of Pure and Applied Coordination Chemistry (SPACC) in Kagoshima

　　　 Power of Live-imaging, Seeing is Believing－Relation between NSAIDs induced small intestinal injuries

　　　 and Reactive oxygen species －
Publication

2022　Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

　　　 Volume 1868, Issue 4, 1 April 2022

　　　 Morphological and functional adaptation of pancreatic islet blood vessels to insulin resistance

　　　 is impaired in diabetic db/db mice

　　　 https://doi.org/10.1016/j.bbadis.2022.166339

2020　Pharmaceutics, 2020; 12(6): 525

　　　 Elucidation of inverse agonist activity of bilastine.

　　　 Hiroyuki Mizuguchi, Tomoharu Wakugawa, Hisato Sadakata, Seiichiro Kamimura, Mai Takemoto,

　　　 Tomomi Nakagawa, Masami Yabumoto, Yoshiaki Kitamura, Noriaki Takeda, Hiroyuki Fukui.

2018　Biomaterials 167 (2018) 143e152

　　　 Novel angiogenesis therapeutics by redox injectable hydrogel - Regulation of local nitric oxide

　　　 generation for effective cardiovascular therapy.

2017　JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2017

　　　 High glucose-mediated overexpression of ICAM-1 in human vaginal epithelial cells

　　　 increases adhesion of Candida albicans.

2017　Evidence-Based Complementary and Alternative Medicine Volume 2017, Article ID

　　　 3620130, 7pages

　　　 Effect of Keishibukuryogan, a Japanese Traditional Kampo Prescription, on Improvement of

　　　 Microcirculation and Oketsu and Induction of Endothelial Nitric Oxide:A Live Imaging

　　　 Study.

2016　Calcif Tissue Int. 2017 Mar; 100(3): 286-97 (Epub 2016 Nov)

　　　 Bazedoxifene Ameliorates Homocysteine-Induced Apoptosis and Accumulation of Advanced

　　　 Glycation End Products by Reducing Oxidative Stress in Osteoblastic MC3T3-E1 Cells.

2016　J. Clin. Biochem. Nutr. 2016 vol. 58 (3) :167–173

　　　Kangen-karyu raises surface body temperature through oxidative stress modification.

2014　Advances in Microbiology Vol.4 No.11, September 2014.

　　　 Clarithromycin Attenuates the Bronchial Epithelial Damage Induced by Mycoplasma

　　　 pneumoniae Infection.

2014　J Physiol Pharmacol. 2014 Jun;65(3):435-40

　　　 Indomethacin-induced generation of reactive oxygen species leads to epithelial cell injury

　　　 before the formation of intestinal lesions in mice.

2014　J Clin Biochem Nutr. 2014 Jan;54(1):12-7.

　　　 Mitochondrial reactive oxygen species accelerate gastric cancer cell invasion.

2012　Gastroenterology. 2012 Oct;143(4):1027-36.

　　　 An orally administered redox nanoparticle that accumulates in the colonic mucosa and

　　　 reduces colitis in mice.

“Life in action”

Our “live imaging” is dedicated to conveying the wonders and motion of life.

By providing further insight into the phenomena of life and by making greater advancements in medicine and science, we are confident that we can positively contribute to the future of human beings.

About TLV

Image Production

The collaboration of our professional staff provides you products of the highest quality.

TLV is different from the rest due to a highly trained, professional staff in medical and life sciences.

We produce images that are both scientific and engaging.

Create through Connection

Research Group

Our research group has developed its own unique technique for visualizing life phenomena under a microscope. We can also handle various objects such as living bodies, cells, bacteria, fungi, and others.

Imaging Group

The imaging group is responsible for capturing the microscopic world through various microscopes and imaging devices. Besides being professionals in imaging microscopy, they also film on location or in the studio.

Direction Group

By assimilating and analyzing the content using their expert knowledge, our direction group delivers the final product as a story that is both easy to understand and intriguing.

CG Creation Group

TLV’s computer graphics (CG) developers then create new CG that combine the reality of representation with effective design through their examination of actual life phenomena.

The Flow of Our One-Stop System for Imaging Production

Planning
Experiment design
Scenario / CG storyboard creation
Experiment / Imaging microscopy
CG production
Filming on location
Editing
Sound recording
Delivery

Tailored to All Needs

Production is conducted with careful consideration so that the expressions used are appropriate for its target audience, which might include doctors, other medical professionals, patients, in-house training participants, or even general consumers. For example, the product can include commentary on cutting-edge information with experiment footage and realistic CG, explaining medical information in an easy-to-understand manner with simple illustrations, using drama to grab the viewer’s attention.

Our versatility can answer your every need, whether it is displaying a particular phenomenon under a microscope, visualizing a thesis experiment with microscopic images, adding more impact to an otherwise straightforward presentation of data introduction, or using microscopic images during lectures.